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The sample volume requirement is less than 150 μl and can be reduced significantly with optimized cartridge design.
The easy method of fabrication and the small sample volume requirement together with the high sensitivity towards uric acid measurement make this biosensor advantageous over the recently reported uric acid biosensors (Table 2).
The reported value for L corresponds to a sample volume requirement of 3 nL per assayed analyte in a 10 μm deep and 100 μm wide microfluidic channel, which is nearly 3 orders of magnitude smaller than that required on microarray based platforms.
Samples for T were assayed in duplicate DSL-40000), whereasampleses for E2 were determined in singleton (DS-43100), owing to the larger sample volume requirement of that assay.
The developed method uses a single sample with low sample volume requirement, generating reliable and precise results without interferences.
The low sample volume requirement of CE and its direct MS coupling make it an attractive alternative for AEC-MS for T6P analysis in plant extracts.
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Additional advantages to the microbiocidal assay are its simplicity, short duration, small sample volume requirements, and that it requires only a minimal amount of specialized equipment to perform.
Ideally, new technologies will reduce sample volume requirements, lessen the amount of time required for microarray analysis (which now averages 24 hours), and reduce microarray costs to the extent that they can be used routinely in the clinic.
The sensor element design has certain advantages over existing SPR sensor configurations: (i) simplified fabrication; (ii) minimized sample volume requirements, and (iii) application to high throughput SPR analysis.
Lower sample volume requirements and reduced user intervention time compared to the manual instruments have improved turnover of calorimetry experiments in a high-throughput format where 25 or more runs can be conducted per day.
Besides, the parallel plate geometry of the apparatus utilized here offers a distinctive edge over Couette type cells used for similar purposes as the latter is often not suitable for studies on well-entangled concentrated polymeric systems due to high viscosity and associated large sample volume requirements.
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