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Only, no split sample verification could be administered because insufficient blood had been drawn.
Considering the mean concentration time of the basins of the study and the target application on flash flood forecasting, the skill of the flow routing simulations is significantly high for peakflow and timing of peakflow estimation, and shows consistency as indicated by the large sample verification.
To characterize the accuracy of these self reported histories, medical record review for a sample of Study 2 cases resulted in verification of the self reported MI status for 98% of the sample (verification by electrocardiogram, cardiac enzymes or imaging).
Obtaining enough material is a challenge at these embryonic stages, particularly from the forelimb bud which contains about 1000 Pax3 positive cells at E10.5, so that it was necessary to prepare material from >100 embryos with each genetic background in order to have enough material for cDNA synthesis, sample verification (see Additional file 1 Figure S1) and triplicate Affymetrix chip analyses.
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SL performed the pathological determination of the samples (verification).
The methylation status of the coding strand was determined for all samples; verification of the methylation status on the noncoding strand was performed for a subgroup of genomic DNA samples and found to be consistent in all cases (data not shown).
Included in the ICPMS analytical runs were the following quality control samples: calibration verification (every 10 samples), reagent blanks (every 10 samples), method blanks (one per batch), matrix spikes (10% of samples), sample duplicates (10% of samples), and NIST SRM 2709 (one per batch).
Additional file 1: Sample identity verification.
In 93 investigations (50.0%) it was clear that the whole sample received verification using the reference standard.
Sample size verification was obtained using one-sample inference for a binomial proportion [ 18] with estimates of NHP use at 60% in the study population and general population use being 46% [ 19], alpha of 0.05, and statistical power of 80%.
The focus on English may also have led to a biased sample as verification of eligibility often relied upon publication in peer reviewed journals and review of conference proceedings, both of which are biased towards English-language publications.
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