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The manually prepared libraries exhibited greater between sample variation than the libraries prepared using the automated protocol.
(b) Between sample variation within a brain region and sample type, as a function of methylation level.
The sample variation was also interrogated on the spatial level by investigating gene expression averaged over ten spots in an inflamed region (Fig. 6a,b).
Once again, we were unable to identify DEG that might serve as useful biomarkers of remission (CRM), given the inter sample variation.
We next adopted the sample weighting method recently reported by Liu et al.21 to reduce inter sample variation prior to DEG identification to improve DEG analysis.
Statistical power in temporal or spatial studies is determined by the random/unexplained sample variation.
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Any sample-to-sample variation between the replicates could then be attributed to the robot.
Ziller, M. J. et al. Genomic distribution and inter-sample variation of non-CpG methylation across human cell types.
In this study, by choosing a mouse model to control sample-to-sample variation, we successfully concluded that the diversity of MSCs is influenced by tissue origin.
Sample-to-sample variation was investigated by small red triangles assessing the libraries at two points during the library preparation process.
Supplementary Figure 1 Analysis of inter-sample variation in the GEP-NET expression dataset and reliability analysis for different interactomes as models for GEP-NET.
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