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Some larger-capacity cookers with nonstick bowls still managed to scorch or "mat" the bottom of a sample using one cup of uncooked rice.
An additional RT-negative reaction without adding the transcriptase was run for each sample using one third of RNA input and volumes.
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One μg of total RNA was used to generate a cDNA from each sample using one-step RT-PCR kit (Qiagen) and custom primers.
The leaf tissue mRNA samples were sequenced on the 454 Titanium FLX platform and each sample used one half picotiter plate.
Simandoux made measurements on only four synthetic samples using one type of clay (montmorillonite), and the samples used had a constant value of porosity.
A common approach is to profile biological samples using one or more omic technologies and then to search for differences in measurements between the sample groups.
Then single-stranded cDNA was generated from the two RNA samples using one of two P-element-specific primers (P4 or P15).
Genomic DNA was removed from total RNA samples using one treatment of Turbo DNase (Ambion) according to the manufacturer's recommendations.
Here, we cluster samples using one gene at a time, much like the classification of samples as ER+ or ER-, ERBB2+ or ERBB2-, etc., utilizing only genes that define distinct subtypes in multiple patient cohorts.
Table 1 outlines the numbers of HapMap SNPs that are called with ≥10× coverage in more than 50%% of samples using one WES capture platform in current use (Agilent V5 + UTR).
Indeed, our results suggest that the detection of senescent cells in cancer samples using one or more of our markers could be used to predict survival in breast cancer, and perhaps also in other types of tumours.
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CEO of Professional Science Editing for Scientists @ prosciediting.com