Sentence examples for sample under comparison from inspiring English sources
Equal amounts of protein are used for each sample under comparison so that any differences in relative peptide/protein amount measured by mass spectrometry reflect differences between samples, not starting protein amounts.
GAGE [ 18] was applied to infer the most differentially expressed pathways or gene sets between the BMP6 added or withdrawn samples under comparison at different time points.
The individual replicates of the samples under comparison are taken together, normalized and the mean relative OD per time point is calculated.
Scatter plots were also generated using this software to inspect the reproducibility of the replicates as well as the degree of variations of the samples under comparison.
When studying mixed cell populations, gene expression profiling can successfully detect differential gene expression in specific cell types present in the samples under comparison.
To avoid false negative results due to inconsistencies in sequenced loci between samples, we report only on those loci completely covered by at least five reads in both samples under comparison.
The ratio controls (which are detected using 'spikes' of different ratios of probe between the two hybridization samples under comparison, see below) measure different ratios of gene expression, over a range of 2.5 orders of magnitude.
Standard selection criteria to identify differentially expressed genes were (1) absolute log2 fold change ≥1; (2) false discovery rate of <0.05; (3) the intensity difference between two samples under comparison ≥1000; (4) individual intensity ≥500.
For each experimental series (i.e. L1 and iL3, and L2 direct and L2 indirect) the amplification of two control genes was used to confirm that equal amounts of cDNA were present in the respective samples under comparison.
The relative intensity of each reporter ion in that cluster is then used to give a measure of the relative abundance of that peptide between the six samples under comparison.
In the current study the maximum fold change was set to 100, meaning that any protein showing a protein ratio of 100 was not detected in one of the samples under comparison.
