For the South Asian immigrant sample, three additional variables of acculturation were examined: years since immigration (0 9 years, 10+ years), age at time of immigration (≤17 years, 18+ years), and language of CCHS interview (English or French, non-official language).
For some of the samples, two additional PCR assays representing the gltA gene and ompA genes were used as well (Table 2) [33], [33].
In the process of amplifying the complete PBoV sequences in various samples, two additional samples (6V, 7V) were positive for 2,407 bp and 2,434 bp amplification products, respectively, which included a partial NP1 gene and complete VP1 gene.
For samples with invalid results on the initially retrieved samples, two additional cryotubes were retrieved, processed and assayed for a second and final attempt.
The copro-antigen tests confirmed all Cryptosporidium microscopy-positive samples and two additional samples were found positive in the Cryptosporidium antigen ELISA.
Thus, we used these gene signatures to classify clinical samples in prostate cancer samples and metastatic tissue samples in two additional datasets.
A second randomly chosen sample was also fully sequenced, and showed a derived allele at position 8814 as in the previous sample, plus two additional mutations at positions 6335 and 8277.
Moreover, the spectra of the free PVDF samples show two additional peaks at low frequencies which are associated with mechanical relaxation processes.
We analyzed the data using a population PK analysis approach and included samples from two additional pediatric trials.
