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The optical microscopy images of the typical sample transferred to the SiO2/Si substrate are shown in Additional file 1: Figure S4.
Excited by X-ray with W as the target, the pure product showed intense broad emission with the peak at about 400 nm suitable for modern photoelectric multiplier tube (PMT), while the Eu3+ doped sample transferred the incident energy to Eu3+ ions and gave strong 5D0→7Fj (j=0, 1, 2 and 4) emission, which fitted for imaging and labeling measurements.
The polymer coating procedure, illustrated in Fig. 4, was: (a) sample dipped in PES/NMP solution for 1 min; (b) sample transferred to a beaker of water in which the NMP solvent is extracted from the PES phase to the water phase to leave a solid PES film on the rock, and (c) PES-coated rock removed from the water and dried in air.
To prepare protein extracts, OTC was dissolved in PBS and the tissue sample transferred to lysis buffer (10 mM Tris (pH 8), 150 mM sodium chloride, 0.5% deoxycholic acid, 0.1% sodium dodecyl sulfate, 1% triton-X100) and protease inhibitor cocktail (Roche).
All spectra were recorded immediately after sample transferred to the cuvette and baseline-corrected with the respective solution without protein.
One milliliter of deionized water was added and the sample transferred to a tube with a Teflon screw cap.
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For all types of produce, regardless of the material and sharpness of the knife used, more than half of all samples transferred viral particles to the knife.
All samples were stored at 4°C and the following day RNAlater® was removed and samples transferred to -80°C until further qPCR processing.
Western blots were performed as previously described using 4 12% gradient gels (Invitrogen, Carlsbad, CA) with samples transferred to PVDF (Millipore) [37].
Samples transferred from Tokyo to Beppu remained frozen while in transit.
After washing with PBS, the needles were detached and the samples transferred to 1.5 ml microtubes for subsequent staining.
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