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Recognizing structural distortions indicative of disease is a manual pattern recognition process that matches patterns in the tissue sample to standard patterns.
In this method, the first step is to apply a clinical sample to standard culture media to see what grows [ 19].
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Concentrations were derived by comparing Ct values of samples to standard curves.
Percent input values were calculated using Rotor Gene software by comparing Ct values of samples to standard curves established by the Ct values of total chromatin controls.
Copy number was calculated by referring the Ct values for each sample to a standard plasmid curve.
Serum Sema3E concentration was determined by comparing the optical density (OD) of each sample to the standard curve.
12 Add 4 7 tubes of radiolabeled samples (standard samples) to measure.
Add 4 7 tubes of standard samples to measure.
12 Add 4 7 tubes of standard samples to measure.
To determine the amount of purified JFH-1 virions, the pUC18-JFH1 plasmid was used as a standard sample to generate a standard curve ranging from 103 1011 copies/mL.
No significant fluorescence signal was observed for the duplicated negative control (NTC) and 26 negative samples (compared to standard positive samples).
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