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Raman spectroscopy was carried out using a Jobin-Yvon T64000 Raman Spectrograph with a wavelength of 514.5 nm from an argon ion laser set at a laser power of 0.384 mW at the sample to minimize local heating of the sample during the Raman analysis.
Four amplification reactions were performed in parallel for each sample to minimize spurious amplification artifacts and purified using QlAquick spin columns (Qiagen) and the products were combined.
The fluorescence intensity images were then normalized by the square of the excitation laser power, at the sample, to minimize day to day fluctuations in laser power.
Thus, the spectral count for all identified host proteins was subtracted from the overall spectral count of the sample to minimize competition effects.
In the prior study [16], we went to great lengths to produce ecologically relevant moral stimuli and matched non-moral stimuli and also to select a professionally homogeneous sample to minimize the effects of different training histories.
A blank was processed in between each GC/MS sample to minimize cross-sample contamination.
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Blanks were injected between samples to minimize carry-over.
WiseMAC is based on the preamble sampling to minimize idle listening and save power.
The samples were collected through grab sampling from different locations, mainly from the mid span and end terminals of the rotary drum composter with a compost sampler to minimize disturbance of the adjacent materials.
OED selects the most informative samples to minimize the covariance matrix of the parameters, so that the expected prediction error of the parameters, as well as the model output, can be minimized.
Mineral oil was layered on the tops of the samples to minimize evaporation.
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