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However, the sample of only 230 women from three select hospitals, although not insufficient but a small sample to do a more robust statistical estimates, is one of the major limitations of our study.
We did not attempt to culture the Trichococcus spp, as there was only sufficient sample to do a gDNA extraction for 16S rRNA PCR but not for culture work.
The data produced from the mixed samples were used to construct the whole transcriptome file, and meanwhile, they were used as the reference and combine with data from each separate sample to do further gene expression analysis.
At last, in order to do the comparison at the same sequencing depth, 18,808 effective bacterial sequences were extracted from each sample to do the downstream analysis.> In order to compare the bacterial species richness among these samples, operational taxonomic units (OTUs) were determined for each sample at distance levels of 3 and 6%% (Table 1).
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What they say to you is "Well, you don't have the writing samples to do that".
The rampant looting of meteorite sites and skyrocketing prices for the fragments, he said, "dramatically reduce who can get samples to do the research".
I chose ten samples to do the pilot analysis.
We demonstrate the feasibility of using "umbrella sampling" to do Monte Carlo Markov-sampling runs each covering a substantial range of density: "density-scaling Monte Carlo," or DSMC.
However, we did not have enough elapsed time for sampling to do this accurately see the Results section and Table 4.
George Church of Harvard University says his project to sequence the DNA of thousands of people called the Personal Genome Project is collecting data from tissue samples to do similar kinds of work.
This involves library work, buying supplies, organizing travel, gathering feedback from colleagues about the types of sampling to do, and--of course--planning the experiments themselves.
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