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Of those examined, enterococci was most predictive; of the 129 samples positive for Salmonella, 88% (113/129) were detected when enterococci were above EPA single sample threshold (61 CFU 100 ml-1); and of the 96 samples positive for Campylobacter, 90% (86/96) were detected when enterococci levels exceeded this level.
For a given cDNA sample, threshold cycle (Ct) values were determined using the Advanced Relative Quantification algorithm for each target gene (Cttarget) as well as Gapdh and ß-actin endogenous reference genes (Ctreference).
For purposes of quality control, 95% of sample threshold and 95% genotyping success threshold were used.
Sample threshold cycle (Ct) values were used to calculate the number of cell equivalents in the test samples.
Ct is the Real-Time PCR sample threshold cycle and ΔCt is (Ct CTCs)- Ct(PBMCs)), PBMCs are the Peripheral Blood Mononuclear Cells (the CTCs-depleted fraction).
We also set a "high quality" sample threshold of 2p-RSE ≤ 0.18, which corresponds to a per marker FPR50 of approximately 1.5%.
Similar(49)
Comparatively, Escherichia coli concentrations were above EPA single sample thresholds in 38% (49/129) of the positive Salmonella samples.
The parameter setting of modified K-means algorithm is as follows: input 45 samples, threshold ε = 0.001, and cluster numbers z = 9.
Approximately 85% of the 47,123 PDB ligands have an RMSD cluster value of ≤ 1.0 Å. Figure 3 RMSD cluster conformer model sampling threshold.
The parameter setting of traditional K-means cluster algorithm is as follows: input 45 samples, threshold ε = 0.001, and cluster numbers z = 9.
The top 26 miRNAs separated almost perfectly the basal-like and luminal-A samples (Threshold number of misclassification (TNoM) ≤6, see Materials and Methods).
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