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However, given that we measured the presence or absence of Campylobacter on chicken products, rather than the quantity of Campylobacter on a given sample, this variation likely did not affect the overall outcome of the study.
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Ultimately, the total number of plots will depend on the variation within the area of interest and how comprehensively the sample describes this variation (Junttila et al. 2008).
Combining data from multiple samples masks this variation.
As KIM-1 appear to peak later than other UBs (approximately 48 hours [ 14]), it is possible that differences in timing of urine sampling explains this variation.
To investigate whether this large sample-to-sample variation in deviating exon usage amounts was cancer specific, comparisons were made between 13 paired CRC and normal colonic mucosa samples from the validation series.
Analysis of 96 samples in parallel greatly reduces sample-to-sample variation and can be completed in one day.
This relatively simple experiment clearly demonstrates that sample-to-sample variation can be detected.
Data was interpreted using BeadStudio and quantile normalized to adjust for sample-to-sample variation.
To correct for sample-to-sample variation, Gapdh served as an internal reference.
Several samples would minimize this variation but this approach has practical limitations since tissue preservation is important during revision surgery.
The prevalence of each condition was highly dependent on the sampling method, and this variation in prevalence had a significant effect on both kappa and PABAK.
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