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The total concentration of mouse IL-18 for sample then is determined by multiplying the value read from the standard curve by the dilution factor for the sample.
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Developed by Professor David Gracias at Johns Hopkins University, these starfish-shaped robots are able to enter the body, collect a minuscule tissue sample, then be extracted by a doctor.
Families who provided DNA samples, then, are required to fill out a form that asks whether they want to be notified when additional tissue samples are identified.
Samples then were melted at 2 8 °C.
Samples then were centrifuged at 8000×g for 10 min.
Culturing the samples then was performed at our certified laboratory.
Samples then were stored at 4°C or -20°C until cloning.
Study samples then were created using a similar procedure to that employed for the simulated data.
The blood samples then were sent by overnight mail to the Institute of Community Medicine at the University of Tromsø, Norway.
Samples then were frozen in liquid nitrogen vapor phase at about -130°C for long-term storage.
DNA samples then were identified with serial numbers and stored at −80°C until subsequent analyses.
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