Sentence examples for sample the membrane from inspiring English sources

In a control sample, the membrane potential (Δψm) was dissipated by addition of 8 µM antimycin A, 0.5 µM valinomycin, and 2 µM oligomycin.

This process would be facilitated by an intrinsically dynamic lateral gate in Sec61 that allows the polypeptide segment inside the channel to reversibly sample the membrane.

In the presence of pathological levels of CRP in the sample, the membrane appears red-brown with color intensity proportionally to the CRP concentration.

To ascertain comparative expression and equal loading of the protein samples, the membrane stained earlier was stripped and reprobed with β-actin antibody (Abcam).

The higher signal in the filtrate compared to control AβO sample (not subjected to filtration) is likely due to an error associated with volume correction after concentration of the sample on the membrane during centrifugal filtration.

By forced transport of the test sample through the membrane loaded with excess autoantigen, antibody antigen complex formation is complete in less than 3 min.

In the first one, a hybridization step is required upon running the sample through the membrane [ 11– 15].

The total protein sample and the membrane fractions were then analyzed by 12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting following standard procedures [ 26].

To ensure equal loading of the samples, the membranes were re-probed with a GAPDH antibody (Cell Signaling Technologies, Danvers, MA, USA).

Following sampling, the membranes were transferred to a Growth Cassette and introduced to the Growth Direct System.

To verify equal loading of samples, the membranes were subsequently incubated with monoclonal antibody β-actin, followed by an HRP-conjugated sheep anti-mouse IgG.