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This design has an expected sample size of 6286%6% of the fixed sample value) and an expected study length of 3.1 years (77% of the fixed sample value).
The expected sample size under H0 is 53.2 (or 74% of the fixed sample value).
The fixed samples were concentrated to 20 30 ml.
The fixed samples were embedded in paraffin.
The fixed samples were decalcified using Cal-EX (Fisher Scientific), and embedded in paraffin for sectioning.
The fixed samples were washed in PBS containing 1% Triton X-100 (PBST).
The fixed samples were embedded in paraffin and serially sliced into 5-µm sections.
The fixed samples were homogenized using Precellys device (Bertin Technologies).
The sample was fixed to the lower table of the machine, using a vice holding the body of the scapula.
A similar approach was used to keep the sample fixed in the x- y plane.
The sample is fixed on the base with adhesive tape so that the sample cannot move relative to the base.
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