These experiments were conducted in two groups of samples, that is those derived from patients who had demonstrated either in vitro and/or clinical resistance to fludarabine (20 patients) and CLL samples that retained sensitivity to fludarabine (20 patients) to assess the drug interactions in each cohort.
In progression to the later grades the IDH1 positive sample split into two categories; those samples that retain a very similar methylation profile after progression (P5, P8, P10, P11, P15, P17) and those that demonstrate a partially remaining CIMP+ve between early and late lesions or greatly reduced (becoming CIMP-ve) degree of methylation after progression (P1, P7, P12, P13, P14, P18, P20).
Slow pyrolysis at a temperature of 400 °C was utilized to generate a biochar sample that still retained some oxygen functionality (which is generally lost at higher temperatures), while ensuring that full pyrolysis occurred, instead of torrefaction which happens at lower (~ 300 °C) temperatures (Shankar Tumuluru et al. 2011).
For the Ewing Island core we reconstructed local fire history following standard charcoal-analysis procedures (Whitlock and Larsen 2001), counting all charcoal particles present in a 1 mL sample that were retained on nested sieves of 125 and 250-µm mesizeize.
A hierarchical loglinear analysis of gender and centre effects on the subtype frequencies in the sibling sample resulted in a model that retained main effects and two-way interactions, but no three-way interactions.
The re-sampling was performed in a manner that retained the structural consistency of each subgroup.
A sample was filtered through a 0.45-μm millipore cellulose filter that retained the hyphae.
We examined the proportion of cells that retained viability and adherence in a subset of samples (n=3).
The 40 genes can be divided into those that generally retain renal cancer-specific expression among all samples, and those that retain it only in the subset of samples (samples 1 to 3, indicated in blue in Figure 3a).
Furthermore, tumour samples that have retained both chromosomes demonstrate elution profiles almost identical to the corresponding blood DNAs.
