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In the OTU-based hotspot analysis we calculated the number of unique eSNaPD assigned OTUs found in each sample that map to a specific gene cluster.
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The fourth column is the total number of paired-end reads from each mouse forebrain sample that mapped to the T. gondii genomic reference file.
For each sample, transcripts that mapped uniquely within 1KB of a gene annotation on the reference genome were attributed to that gene symbol, leaving ∼27K unique genes with at least 1 read across the 25 sample-preparation type combinations (14 FFPET and 11 frozen samples) in the 3SEQ analysis.
Unfiltered BAM files were used to estimate the proportion of reads in each sample that mapped to the expected target organisms using samtools flastat (version 0.1.19-44428cd) (Li et al., 2009).
The percentage of clean reads (144.4 146 bp average length) from each sample that could be mapped ranged from 74.76 to 89.35% (Additional file 2: Table S1).
Immunoprecipitated DNA was then prepared for sequencing using the Illumina ChIP-seq library kit, and DNA libraries sequenced using an Illumina HiSeq2000, which generated approximately 160 million reads per sample that were then mapped to the M. smegmatis genome using Bowtie [ 33].
The lower bounds on a sample that uses a Likert mapping will need to be many times as large as one that assumes a full Gaussian belief distribution, as in (Crook and Good 1980).
Thus the lower bounds on a sample that uses a Likert mapping will need to be ( hat{omega} ) times as large as one that assumes a full Gaussian belief distribution.
The content of a table entry is the centroid of the input samples that are mapped to this entry.
We obtained an average of 35.3-million reads from individual RNA-Seq samples and an average of 19.4-million readseads from individual ribosome profiling samples that were mapped to human and EV71 genomes.
Raw sequence data were aligned to the Trypanosoma brucei brucei 927 genome, and reads per kilobase per million mapped reads (RPKM) were calculated for each CDS for each sample replicate, including only reads that map to a single location in the reference genome.
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