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We found one saliva sample that demonstrated low concordance (82.6%) with its replicate blood sample.
Pathologic diagnosis was attained in some nodes only through cell block processing in 40 patients (14.8%), and cell block was the only sample that demonstrated mediastinal metastases in 7 of them.
as a housekeeping gene from each sample that demonstrated proportional β-actin expression to the amount of total RNA as a control for any inherent variability of the reverse transcription reaction [44].
The sample that demonstrated strongest pAktser473 signal contained both normal and cancerous tissue.
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Very low band intensities were found in the kidney samples that demonstrated considerably reduced Dnase1 mRNA levels and Dnase1 enzyme activity (Figure 1D, lanes 10 12), whereas mice with normal mRNA expression and mesangial matrix deposits only, had Dnase1 protein expression comparable to the normal control and pre-proteinuric mice (Figure 1D, lanes 7 9).
For those samples that demonstrated major antiretroviral resistance mutations as defined by the International Aids Society-USA Drug Resistance Mutations Group [27], stored plasma from their first cycle was sent for HIV genotyping to determine whether the mutations were present at baseline or had developed during the course of the study.
We carefully evaluated the results of each assay and only included those samples that demonstrated positive signals in most assays.
The rare samples that demonstrated a significant second peak in the melting profile were not used in analysis.
In order to test this, we compared miRNA activity patterns between the sarcoma samples that demonstrated RAS pathway activation to those that did not.
The rAY was pre-incubated with recombinant YKL-40 overnight before applying to breast cancer samples that demonstrated strong expression of YKL-40 in a pretest.
Any samples that demonstrated "aging peaks" on high-performance liquid chromatography, evidence of degradation during storage and/or shipment, were considered unacceptable for analysis.
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