Each sample was subsequently quantified in triplicate using fluorimetry (employing PicoGreen® dsDNA quantitation reagent, Cambridge Bioscience, UK) and samples that were accurately quantified (± 0.5 ng/μl) were accepted for pooling.
The use of growth kinematic profiling to create tissue samples that are accurately positioned along the expansion growth continuum of Arabidopsis inflorescence stems establishes a new standard for transcript profiling analyses of such tissues.
In this study, we have applied growth kinematic profiling (GKP) to a series of individual Arabidopsis inflorescence stems as described in Hall and Ellis (2012) [ 15], and used the resulting growth rate profiles to generate pooled samples of stem tissue that accurately represent four discrete growth stages along the cell wall expansion developmental continuum.
There is, however, concern that this practice introduces added experimental error, as nitrogen vs helium mobility measurements differ substantially in magnitude, and the success of calibration strategies relies heavily on careful selection of calibrants that accurately describe the sample conditions, charge state, mass range, and chemical class of the system of interest.
The description in Table 2 gives four baseline statistical components, where TP and FN denote the number of positive examples which are accurately and falsely predicted, respectively, and TN and FP represent the number of negative samples that are predicted accurately and wrongly, respectively.
Self-observation and experience sampling methodology [ 9- 11] was used to determine that we were creating a taxonomy that accurately reflected chaplain activities.
As such, even fixations with high spatial precision can have few samples that were not accurately recorded and, consequently, will generate the same peaks in velocity as saccades.
But no one can do that accurately.
That accurately reflects your situation.
Geologists have long sought tranquillityite in Earth rocks, partly because studies of lunar samples suggest that accurately measuring the proportions of radioactive isotopes in the mineral could be used to ascertain the age of the rocks.
