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Multimammate rats were the dominant commensals at all sampling locations, comprising 64.5% of the overall sample (Technical Appendix Figure).
This reaction was performed in triplicate for each sample (technical replicates).
For each cDNA sample, technical duplicates in each of three biological replicates were tested.
Nevertheless, there are 14 unique ospA haplotypes and 14 unique ospB haplotypes in this sample (Technical Appendix).
Similarly, negative two was added to the specified set of genes and samples that were simulated as overall sample technical outliers.
According to these values and the hematocrit of the sample (Technical Appendix), the global infectious titer whole blood in the tested patient would be ≈4.45 ID/mL.
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Data reliability was assessed by checking Pearson correlation coefficient C among samples: technical replicates resulted more correlated among themselves (C>0.96).
However, systematic inter-sample technical artifacts caused by variability in sample preservation, bio-molecular extraction and platform fluctuations must be removed to ensure robust data.
Preprocessing algorithms must contend with differing probe hybridization efficiencies that result in greater inter-probe than inter-sample variability, probe intensity variances that change with intensity levels, and inter-sample technical error.
Amplification was positive in 19/85 (22.4%) samples; sequences were obtained for 18 of these samples (Technical Appendix).
For 3 of the samples, technical replicates of the capture and sequencing were performed.
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