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Homogenized samples were 10 fold serially diluted in sterile saline up to 108 and serial dilutions (dependent on sample) spotted (50 µl) in duplicates onto CHAB quad plates, with plates allowed to dry without spreading.
An alternative method of sample collection is the use of dried urine spots (DUS), that is, a urine sample spotted on blotting paper, which allows for stabilization by drying.
The Aushon 2470 Arrayer (Aushon BioSystems, Billerica, MA, USA) with a 40-pin (185 μm) configuration was used to spot lysates onto nitrocellulose-coated slides (Grace Bendlabs, Bend, OR, USA) using an array format of 960 lysates/slide (2880 spots/slide) with each sample spotted as technical triplicates including test and control lysates.
Analysis of the radiolabelled liposomes was carried out by ITLC using ITLC-SA (0.75 × 9 cm) with a 2 μL sample spotted at the origin, 1 cm from the bottom of the strip, and run for 8 cm in 0.1 m citrate buffer, pH 5. ITLC strips were scanned on a mini-TLC scanner and analysed with Laura software (LabLogic).
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The HSPA1 staining intensity varied between kidney cortex samples spotted on TMAs.
The same methodology was used with 9 freshly prepared methemoglobin samples spotted on absorbent material.
Prospective participants were prescreened using finger prick blood samples spotted on glass slides by light microscopy.
The solvent penetrates the paper by capillary action and, in passing over the sample spot, carries along with it the various components of the sample.
The IR beam was focused first on a sample spot, then on a clean fiber spot.
However, XRPD is a bulk analysis, while SEM EDX probes merely an arbitrary small sample spot.
The light distribution of the chamber was determined by measuring UV intensity at each sample spot.
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