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Gel scaffold regions were filled with gel solution samples using auxiliary channels.
We will use the Sakai course management system for grades and sample solutions.
Sample solutions will be posted in the Sakai under "Resources". For discussion and Q&A, we will use Piazza.
No adverse effects were observed following administration of any of the sample solutions.
To ensure CO2-equilibration, all sample solutions were measured at least 1 h after preparation.
Test the waters and sample solutions until you find one that works best for you.
Where only small volumes of sample solution exist, vial inserts can be used allowing samples to be analysed from as little as 20uL of solution.
Disposable plastic cuvettes were used with 1.0 mL of sample solution, which was filtered through a 200 nm filter immediately before measurement.
