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This was evaluated by measuring absorbance of freshly prepared standard and sample solutions, and repeating the measurement after 24 h.
In brief, sterile paper discs (6 mm) were impregnated overnight in the sample solutions and then left to dry for 24 h at 37°C in sterile conditions.
Furthermore, we utilize the graph theory technique and the nonnegative semimartingale convergence theorem to investigate the almost sure stability of sample solutions and give the sufficient principles to locate their limit sets, which is helpful to understand the dynamical behaviors.
Furthermore, we utilize the graph theory technique and the nonnegative semimartingale convergence theorem to obtain the almost sure stability of sample solutions and the sufficient principles to locate their limit sets, which correlate closely with the topology property of CSDDEs.
Chromatograms obtained from standard solutions were also compared to those from the sample solutions, and interference was not observed in the presence of formulation excipients, indicating that the specificity of the method was satisfactory Table 5.
In brief, 140 μL of 0.1 mM sodium phosphate buffer (pH 8.0), 20 μL of 0.2 M DTNB, 20 μL of sample solutions and 20 μL of 0.2 M acetylcholinesterase/butyrylcholinesterase solution were added by multichannel automatic pipette (Gilson pipetman, France) in a 96-well microplate and incubated for 15 min at 25 °C.
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Aqueous solutions of dextran (core solution), sodium alginate (sample solution) and CaCl2 (sheath solution) were extruded simultaneously from the inner, intermediate and outer cylinders, respectively.
These channels were used as flow path for the sample solution and a thin compartment for the acceptor phase.
The kit consists of a centrifuge tube (reservoir for the sample solution) and a piece of a porous hollow fiber (for sample extraction).
Some important parameters, including kind and volume of extraction and disperser solvents, pH of the sample solution, salt concentration in the sample solution and extraction time were investigated and optimized.
Polysaccharide extractions by 1) acidic sample solution with agitation, 2) flowing acidic sample solution, 3) static magnetic field combined with flowing acidic sample solution, and 4) rotary magnetic field combined with flowing acidic sample solution were conducted at pH level of 2 4, temperature of 30 50 °C, and treatment time of 0 180 min.
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