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Each sample set was incubated with 10 μg of purified antibody.
A set of 72,200 SNPs segregating in this sample set was used.
Half of the sample set was stored in ambient laboratory conditions while the remaining sample set was subjected to 50 freeze thaw cycles.
The sample set was comprised of ten balm samples from human mummies, three balms from shrews, and ten samples of residues scraped from jars and amphora from storehouses.
The examined sample set was randomly collected and could thus be considered as a representative selection.
The sample set was made up of 44 early-onset (<35 years old) and 54 late-onset cases (>63 years old).
The average DP of the sample set was 1860 for handmade and 1240 for machine-made samples.
At the very beginning, before a sample set was annotated, the annotators revised the guidelines and posed questions to improve the guidelines in a first refinement round.
The overall classification accuracy of the sample set was 86.4%.
The complete sample set was randomized and profiled in two independent iterations.
The median percentage of the genome with copy number alterations in our sample set was 8.4% (range: 0.6 15.1%).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com