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Transcript abundance was expressed as a ratio against a standard comparator sample run with all plates that consisted of complementary DNA made from an ERα-positive endometrial adenocarcinoma Ishikawa cell line (Nishida et al., 1985).
However, a parallel sample run with no SGFRs added showed zero reads mapping to the regions of select fused gene junctions, and therefore the COLO829 can be considered to be a high complexity neutral background sample for this study.
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Furthermore, concurrent sample runs with the ELISA reference method showed 100% agreement.
Shows the number of sample runs with ndSNPQC ≥ 26 pre-validated for each somatic mutation.
Sample runs with spmoB were performed at protein concentrations of 0.3 30 μM.
Of the 85 pre-validated sample runs, the OncoScan® assay detected this mutation with a high confidence call in 76 sample runs, with a lower confidence call in 8 samples runs and as undetected in 1 sample run.
Within the amount of protein loaded on the gel, we detected only a single band in each sample running with the molecular mass corresponding to TBC1D4-short and TBC1D4-long.
The data was collected under validated methodologies with quality control samples run with each batch.
For samples run with FID, compounds had been identified beforehand using GC-MS.
It should be noted that Figure 6 represents all patient samples taken through RT-PCR, including the 23 samples run with less than 1 ng/well input RNA.
The sample run began with the opening of the entrance door.
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