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Following these DNA input guidelines will accordingly reduce the opportunities for human error inherent in manual sample reprocessing and minimize the cost to generate each mtGenome haplotype.
Based on the observation of some PCR failures with positive control DNA (this paper) and blood serum extracts when DNA concentrations were low, and given the extent of sample reprocessing necessary at various PCR input DNA quantities with the blood serum specimens, we suggest an input DNA concentration for PCR of 50 pg or greater when possible.
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Both samples were reprocessed and analyzed each time a given analyte was measured, in all cases z values < |3| we considered, for our purposes, to be satisfactory.
Formalin-fixed, paraffin-embedded (FFPE) tissues were sampled and reprocessed for transmission electron microscopy (TEM) using standard techniques.
The application of micro-electro-mechanical systems (MEMS) to evaluate the chemical properties of radioactive solutions has been investigated with the example of a liquid sample taken from reprocessing plant vessels.
Removal of that sample and subsequent reprocessing of microarray data removed some of these targets from the RMA and PLIER fold change lists.
Electron microscopy performed on one of the biopsy samples, even after reprocessing and reanalysis, failed to show GBM changes typical of NPS.
All reprocessed samples were quantified with the Tophat/Cufflinks pipeline, as were the HPA samples in the published data.
The company hasn't yet commented publicly on the mistake, releasing information only to its customers, reprocessing the samples in question, and considering ways to prevent a repeat.
This would eliminate the burden and expense of reprocessing control samples each time an experiment is repeated.
New aliquots were obtained from the original sample archive and were reprocessed using a different kit lot and sequenced.
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