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"That sample remained in the system for a long period of time," Spitzer said.
At pH 7.4 a self-supporting hydrogel formed, whereas at pH 9 no hydrogel formed and the sample remained in solution as shown in Fig. 6A.
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Continue with whole sample remaining in the centrifugation tube.
(i) Analyze by HPLC as described in Section G. Continue with whole sample remaining in the centrifugation tube.
The temperature was then decreased at 1 °C/s to the next temperature and pressure condition with the sample remaining in the HADC, and the measurement protocol was repeated.
The coarse part of the sample remaining in the beaker was dried completely at 110°C and was weighed to yield the weight, by difference, of the removed fine part (< several tens μm).
Before mechanical testing, each sample (remaining in the culture tube) was rapidly warmed to the testing temperature (22°C) in a 37.5°C saline bath [ 1, 4, 16, 17].
Stability of membership in both low and severe adversity classes was striking with over half the total sample remaining in the same class throughout.
Class membership was fairly stable over time with 55.3% (630) of the total sample remaining in the same class across all time periods (patterns 111, 222, 333, 444).
The ITC sample cell was loaded with 1.5 mL of degassed DmsD sample, and the sample remaining in the syringe after fill was used to confirm the protein concentration with a Bradford assay and absorbance at 280 nm.
Overall CMD prevalence was substantially lower in the follow-up sample remaining in displacement than it had been at baseline, falling from 18.8% to 8.6%; the prevalence was slightly higher (10.3%) in return migrants, although confidence intervals overlapped.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com