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For each sample, quantitative PCR was performed in triplicate using 1.5 ng cDNA in 10 µl reactions composed of 2X-SybrGreen Master Mix (Applied Biosystems) and a final concentration of 0.25 µM gene-specific primers.
To validate the singleton CNVs called by the software packages in the BiGS sample, quantitative real-time PCR (qPCR) with SYBR-green dye was used to measure the copy number of a subset of singleton CNVs using the ABI Prism® 7900HT Sequence Detection System.
Moreover, that test includes an equipment to sample quantitative data.
To quantify Axl and Gas6 mRNA expression levels of each sample, quantitative real-time PCR was performed using the LightCycler thermal cycler system (Roche Diagnostics Japan, Tokyo, Japan).
Further studies that comprise a larger study sample, quantitative type-II collagen and GAG content assessment for further comparison and observations under in vivo conditions with reasonable acquisition times and image resolution are warranted to confirm our results.
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For analyzing the samples, quantitative chemical analysis methods were adopted in the laboratory.
For characterization of the foamed geopolymer samples, quantitative X-ray diffraction (QXRD), thermogravimetric (TGA), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) techniques were used.
In order to obtain high contrast images and detailed descriptions of label free samples, quantitative interferometric microscopy combining with phase retrieval is designed to obtain sample phase distributions from fringes.
That is, given an out-of-sample quantitative outcome vector (y2) and its prediction (y ^ 2 ), the accuracy measure should be such that for any coefficient b > 0 the accuracy of prediction y ^ 2 is identical to that of prediction y ^ 2 ∗ = b y ^ 2.
For some samples, quantitative assessment of IL-1Ra was performed by ELISA (E) or by BIAcore (B) analysis.
To compare protein expression between different biological samples, quantitative proteomics has developed both gel-based and gel-free methods.
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