Exact(4)
For each sample, quantification of cells positive for a given marker was performed by analysis of 5 10 representative regions of the section.
For each sample, quantification of gene transcript levels was performed twice in 96-well PCR plates (ABgene).
After concentrating the sample, quantification of dioxins and dl-PCBs was done using hr-GC/hr-MS.
The residual amount of the probe compound that remained in solution at the equilibrium was quantified using the control sample (" Quantification of the adsorption of probe compounds by SPME fiber" section) as external standard.
Similar(56)
For watershed samples, quantification of total coliforms and E. coli was determined by the Colilert® QuantiTray 2000 method according to the manufacturer's recommendations (Idexx Laboratories, Westbrook, ME).
In this manner, we present a methodology for isolation of individual strains from mixed C. difficile samples, quantification of antibiotic treatments, and the engineering of nutrient environments to control microbiomes.
Normalized data were also used to estimate model-based estimation of sample quantification for individual proteins.
Spore load was quantified in twofold for each sample, and quantification of samples with a difference of more than 1 CT between replicates were repeated, but excluded from further analysis if the difference remained higher than 1 CT.
Recognizing that the quality of genomic DNA could potentially hinder the throughput of mutation detection, we sought an alternative method of sample quantification and normalization.
The number and volume fraction of voids were calculated for each tensile sample, allowing quantification of the evolving ductile damage via the measured increase in average void diameter after each strain increment.
However, current MS methods require relatively large amounts of sample and quantification of phosphorylated sites is challenging, thus new phosphoproteomic strategies are needed.
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