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Sample quantification and detection were performed by gas chromatography tandem mass spectrometry.
GC coupled with tandem MS was used for sample quantification and detection.
Gas chromatography coupled with tandem mass spectrometry (GC MS/MS) was applied for sample quantification and detection.
Results showed that quantitative polymerase chain reaction (qPCR -based sample qPCR -basedion and library normalization and the appropriate number of pooled librariesampleconcentration of diluted libraries provided a greater level of MPS performance and repeatability.
Illumina libraries were clustered for sequencing immediately following sample quantification according to manufacturers recomendations (Illumina).
For each sample, quantification of cells positive for a given marker was performed by analysis of 5 10 representative regions of the section.
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For watershed samples, quantification of total coliforms and E. coli was determined by the Colilert® QuantiTray 2000 method according to the manufacturer's recommendations (Idexx Laboratories, Westbrook, ME).
In the case of glioma tissue samples, quantification was done by using the signal resulting from the known concentration of 2HG-d4 internal standard added to the sample prior to the sample processing and analysis.
The SBV genome was detected in only CNS samples (quantification cycle value 28.8); bovine viral diarrhea/mucosal disease virus and BTV-8 genomes were not detected.
Luminescence was measured with a luminometer (Mithras, Berthold Technologies, Bad Wildbad, Germany) and ATP concentration determined using standard curve allowed samples quantification.
In this manner, we present a methodology for isolation of individual strains from mixed C. difficile samples, quantification of antibiotic treatments, and the engineering of nutrient environments to control microbiomes.
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