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This minimizes the probability of methodological errors and improves sample quality by avoiding the use of previously thawed samples [23].
Scanned GeneChip files (CEL files) are normalized and assessed for hybridization success and sample quality by a proprietary gene expression data quality control system (ResultsPX™), previously described [ 2].
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In the present study we developed a single-step triplex real-time polymerase chain reaction (PCR) assay for detection of Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) with sample quality check, by using β-actin as housekeeping gene.
However, success of these tests entirely depends on sample quality determined by timing and handling of samples [ 1], and a number of outbreaks in the region were not serotyped and/or characterised.
Sample quality control was performed by identity-by-state clustering across all samples to evaluate cryptic relatedness for each sample and by use of principal component analysis to exclude genetically heterogeneous samples from further analysis.
Sample quality was checked by examining the filter cakes under a binocular microscope.
Sample quality was confirmed by gel analysis.
Labeled sample quality was assessed by Nanodrop™ and Bioanalyzer™.
The sample quality was checked by using a Bioanalyzer 2100 (Agilent).
Sample quality was verified by measuring the ratio of 28S to 18S rRNA using the Agilent 2100 Bioanalyzer.
Ten BALF samples of 70 were excluded from the analysis of total Treg due to poor sample quality as assessed by flow cytometry, leaving 60 samples for total Treg analysis (Figure 2).
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