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The use of time-independent sampling produced very different results.

An efficient ultrasonic probe has been used to radiate directly the samples producing very fine emulsions from immiscible liquids.

Individual measures of sampling adequacy (MSA) produced very good results, (from 0.86 to 0.95).

Ethnicity was reported using 11 response options based on 1991 UK census (1991 Census, Crown Copyright, ESRC purchase), but because of the diversity of the non-white groups, subdividing the sample into different ethnic sub-groups produced very small samples, and so ethnicity was dichotomised into 'white' and 'non-white' for these analyses.

In the DNA analysis, blood and normal esophageal samples from a single individual produced very different scores in PC1 (Figure 1C and 1D).

The DNA samples extracted from whole blood produced very low proportion of detectable HIV-1 DNA levels (data not shown), due to the fact that whole blood contains many cell types, and were not used in the analysis.

Of these, 2 animals produced very small samples due to hyperventilation.

Separation of the sample and capture chamber with a polyethylene frit produced very small bubbles, resulting in heavy sample foaming due to minimal surface tension stress lengthening the time needed for the bubbles to burst.

Interestingly, samples drawn from the same animal produced very similar data, suggesting that the main source of this variation is biological variability (see also precision of the miR-34a qRT-PCR of standard 2.46 × 10 05: CV = 10.3%, Table 3).

Sensitivity testing with a longer follow-up period but a more restricted sample (using just the 1978 198282 birth cohorts) produced very similar results.

The two criteria produced very different figures for the probable prevalence of low QOL in this sample.

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