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Sample preparation was based on solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) tandem mass spectrometry (MS/MS) was used to separate and detect the compounds.
Sample preparation was based on our previous work (Pan et al. [2015b]).
Sample preparation was based on solid phase extraction.
Sample preparation was based on the method for cereals described by Sulyok et al. [ 4].
Sample preparation was based on simple liquid-liquid extraction into cyclohexane (1 : 1).
Sample preparation was based on their previous 2D-PAGE studies of tears [ 45].
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Samples from ten fruits were pooled for RNA extraction; RNA and oligo dT -primed cDNA sample preparatioligo dT -primed the procDNAresamplechilmiller et al. [ 42] and Ando and Grumet [ 6].
The procedure employed for sample preparation is based on preincubation of cells with the probes, thereby proving that 1) the new compounds do not need a permeabilisation step, which is necessary for penetration of phalloidin, and 2) cell spreading is not influenced by preincubation with the new systems.
Besides the trehalose sample, preparations were based on common viscoelastic substances known for keeping the moistness, like polyvinyl alcohol, hyaluronic acid, or methylcellulose.
A sample preparation was only based on extraction with methanol water mixture and subsequent filtration of the extract.
To maximize the capacity for long-read sequencing, sample preparation was optimized based on bisulfite conversion and PCR amplification.
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