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For ritonavir, accumulation in PBMC was highly dependent on the sample preparation used.
The MS signal intensity of any compound is affected by the type of sample preparation used and its molecular environment.
The presence or absence of density in the nucleotide-binding pocket is consistent with the sample preparation used for each reconstruction and supports their interpretation.
Because the sample preparation used in this protocol is highly robust and reproducible [ 24], we were able to use equivalent mass inputs of all modified and unmodified targets during hybridization, allowing us to present our results quantitatively rather than qualitatively.
The differences between whole bone elastic modulus values (~ 7 GPa) and matrix level elastic modulus values (~ 30 35 GPa) are in line with the findings of other studies [36] and result primarily from beam theory simplifications at the whole bone level, porosity (included at the whole bone scale but not at the microscopic scale), and the sample preparation used for the nanoindentation protocol.
A key advance facilitating higher throughput and lower costs for several of these platforms was migration from the clone-based sample preparation used in Sanger sequencing to the massively parallel clonal PCR amplification of sample molecules on beads (Roche 454 and ABI Solid) or on a surface (Solexa) [ 3, 4].
Similar(54)
Sample preparation using steam distillation or solvent extraction may also have an influence [8].
We developed a method of sample preparation using epoxy compound, which was validated in two steps.
A rapid and convenient sample preparation using ground tissues allowed a sensitive detection of the herpes-like virus infected oysters.
The sample preparation using a dual beam system is discussed as well as details of the electron optical setup of the microscope.
We then optimized sample preparation using lysozyme and hybridization conditions including temperature, as well as the formamide concentration and the salt concentration in the washing buffer.
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