Sample preparation, treatments, and the experimental setup were similar to those for Experiment 1.
The experimental setup, including sample preparation, treatments, and instrumentation, was the same as that for Experiment 1a.
The extension of SMSI and nickel niobate formation depend on the niobia silica interactions, which are a function of sample preparation and treatment.
However, some other studies demonstrated otherwise [ 18, 19], which may reflect on the precise nature of sample types being analysed, in that optimum selection of reference genes depends on the specific cohort of samples, the method used to retrieve these samples and sample preparation and treatments.
MD handled the sample preparation and their treatment.
Subsequent sample preparation included heat treatment as well as Ar+ ion irradiation under various conditions, which were carried out to modify the fullerene coatings.
The discordance may reflect the difference in sample preparation and patient treatment.
MS carried out the molecular genetic studies of the DKO-SNAP mutants, performed the localization studies including confocal laser scanning microscopy and image analysis, sample preparations and treatments, analyzed the solubility of the AtAPY1-GFP and participated in the drafting of the manuscript.
For gene expression microarray analysis following siRNA knockdown of T-UC.300A or ATRA treatment, sample preparation was carried out as previously described [ 19].
For details regarding sample preparation, live imaging, colchicine treatment, data processing and analysis, qRT-PCR, and bioinformatics tools used for GO term, alternative splicing, network, and cluster analyses, see Extended Experimental Procedures.
