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PV performed most mussel treatments and work management, participated in total RNA sample preparation, systematic sequencing of cDNA libraries and design of study.
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LV performed total RNA sample preparation and systematic sequencing of the cDNA libraries.
CDP performed total RNA sample preparation, construction and systematic sequencing of the cDNA libraries, annotation of ESTs, qRT-PCR and drafted the manuscript.
Various kinds of effects on the characteristics of the claystones are highlighted and discussed, and the procedures for core extraction and packaging as well as a systematic sample preparation protocol are proposed in order to minimise the uncertainties on test results.
We have developed two sample preparation methods and a systematic algorithm for class I HLA typing using next-generation sequencing.
The NormFinder approach stands out because it makes a balance of two sources of variation, but it does not account for systematic errors during sample preparation.
However, the technology also brings with it new issues such as the requirement for large amounts of starting material, cumbersome library preparation; novel systematic biases during sample preparation and sequencing that must be accounted for when analyzing the data.
There have been substantial recent developments in sample preparation and MS methods to allow systematic analysis of glycopeptides derived from biological samples, and quantification of their glycan related isoforms, thus yielding identification of specific glycoforms associated with cancer as potential biomarkers.
All arrays were thus normalized to correct for systematic difference due to sample preparation.
This approach eliminates bias which might be introduced when solely using control transcripts and it prevents systematic errors introduced by the sample preparation method.
Thus, metabolic labeling reduces the systematic errors that may accumulate during sample preparation between the heavy and light samples [14].
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