First, disulfide bond scrambling is prevented by blocking free thiols with N-ethylmaleimide (NEM) and by maintaining an acidic pH throughout the sample preparation process, the latter of which includes precipitating freshly prepared protein samples with trichloroacetic acid (TCA) as early as possible and carrying out all protease digestions at pH 6.5 (Fig. 1).
During the sample preparation process blank controls were included in the pulverization, extraction and amplification steps.
The purification method was simple, efficient and evaded tedious sample preparation process.
Parameters affecting the performance of the sample preparation process were evaluated using experimental factorial designs.
The results demonstrated that the partially reduced GO could be obtained during the sample preparation process.
After the sample preparation process, transmission electron microscopy is performed on cryo samples to generate tilt series of two-dimensional images (micrographs) taken at fixed angular increments.
High reproducibility of the sample preparation process prior to peptides being tagged and combined is of upmost importance for obtaining unbiased data.
Preliminary experiments involved concentration-balancing the SIS peptide mixture to the pooled cosmonaut plasma, performing rigorous interference screening through ion ratio analysis, and automating the sample preparation process on a liquid handling robot.
The thermal reduction of GO during the sample preparation process, the dispersion state of GO in the composites, and the crystallization of PVDF matrix were comparatively investigated.
The new transmission mode desorption electrospray ionization (TM-DESI) technique is not applicable to bulk materials, but instead is a method designed to simplify the sample preparation process for liquid samples and sample extracts.
