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Although the positive ion, non-targeted dataset was not randomised during LC MS collection, the inclusion of QC samples throughout the collection process arguably allows for correction of drift and other systematic noise (e.g. biases correlated with analysis order and/or sample preparation order).
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Sample preparation: In order to initiate liquid liquid phase separation the sample was prepared as reported by Muschol and Rosenberger.[ 42] Briefly, lyophilised HEWL was reconstituted in NaOAc (0.1 m, pH 4.8) and NaCl (5 9 %, w/v).
A UV-ozone cleaning is performed at the end of sample preparation in order to get rid of remaining organic contamination.
This is mainly due to the lack of consistency in providing sufficient data on molecular weight, source of chitosan and sample preparation in order to compare and draw conclusions [ 23].
Light exposure was minimized during sample preparations in order to avoid photoisomerization.
Therefore, a portion of the anhydrous samples was doped with iron (see "Sample preparation" section) in order to decrease of the 29Si T 1.
In the XRD sample preparation process, in order to make the preferential orientation (002) planes on the holder as much as possible, the XRD sample was prepared as follows.
Hollow fiber LPME (HF-LPME) is another easy and low-cost sample preparation method in order to extraction of analytes from complex samples (Shen and Lee 2002; Lee et al. 2008; Saraji et al. 2011).
Exposure to daylight was avoided over the entire sample preparation procedure in order to minimize potential deamination of the target compounds.
In the area of proteomics and genomics, for instance, major opportunities exist for the miniaturization and integration of sample preparation steps in order to handle limited amounts of clinical samples.
The aim of this paper is (1) to establish a sensitive and selective method for the determination of bovine β-casein based on its tryptic signature peptides; (2) to design stable isotope-labelled internal standards; and (3) to optimise the sample preparation method in order to digest the soluble and insoluble β-casein after baking.
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