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Accordingly, the TEM sample preparation methodology needs to be further exploited with and without destructive methods.
To demonstrate that our sample preparation methodology yields PBMCs to a high degree, we performed flow cytometry analysis.
With improvements in system operation and sample preparation methodology, the sensitivity limit of the system is expected to increase to approximately 1×10−15.
By including a separation step prior to the MALDI-TOF-MS analysis and hereby minimizing suppression effects allowed us to obtain higher sequence coverage of proteins compared to conventional MALDI sample preparation methodology.
The results obtained proved the suitability of the combination of MAE with DLLME as a sensitive sample preparation methodology for the analysis of haloanisoles and halophenols in solid enological matrices.
An effective low cost sample preparation methodology for the determination of regulated fragrance allergens in leave-on and rinse-off cosmetics has been developed applying, for the first time, matrix solid-phase dispersion (MSPD) to this kind of analytes and samples.
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Due to reducing size of elementary devices, increasing number of metallization levels and decreasing of power supply voltage, the debug and failure analysis of advanced CMOS designs requires the implementation of specific backside sample preparation methodologies and backside measurement flow.
The merits of different preconcentration methods have been thoroughly discussed by Amaral et al. [24], while Ref. [3] provides an overview of the pros and cons of wine sample preparation methodologies (see Chapter 8). Figure 1 presents some typical 1H NMR spectra of freeze-dried Port wine samples of different age and the more prominent compounds identified in them.
Therefore, the sample preparation methodologies, which have significant effect on these newly identified human and murine immunoreactive proteins of B.p but not on these known pertussis antigens, should be also one of the main factors resulting in the apparent differences between the two immunoproteoms of B.p.
Furthermore, when dealing with on-line sample preparation methodologies like turbulent flow chromatography, factors such as the pH during extraction may be crucial for an efficient extraction procedure.
Two major LBC platforms are commonly used (ThinPrep (Hologic) and SurePath (Becton Dickinson)) These employ different sample preparation methodologies, which may result in different unsatisfactory rates.
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