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The data processing includes a normalization method to reduce the obscuring variation between microarrays, which might be introduced during the processes of sample preparation, manufacturing, fluorescence labeling, hybridization, and/or scanning.
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Although Affymetrix arrays show high precision and repeatability, array results can be influenced by the variation in manufacturing process, sample preparation and data analysis.
Further sample preparation was done as described by the manufacturer (Illumina, Eindhoven, The Netherlands).
Samples were prepared for digestion using the filter-assisted sample preparation method (https://doi.org/10.1038/nmeth.1322).1322
Y.C. assisted in sample preparation.
L.S. participated in sample preparation.
J.M.K. developed sample preparation protocols.
Staff of the CARF assisted sample preparation.
S.V. performed sample preparation and RNA extraction.
Sample preparation and characterization using EM.
M.K. performed sample preparation and imaging.
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