For these measurements, a new sample preparation facility was installed at the INFN-LABEC laboratory of Florence Italyy).
The discrepancy between two methods is due to sample preparations, irradiation facility and geometry.
Imaging with electron microscopy (EM) can provide highly resolved mapping of proteins; however, EM requires complex sample preparation and a specialized facility.
The home-made screens have been tested on more than 200 different protein constructs at the Sample Preparation and Characterization (SPC) facility at EMBL Hamburg.
Two devices that utilise MNP tags are now available, and both are well suited to point-of-care-diagnostics, as they require no specialised laboratory facilities or sample preparation, are inexpensive to use, and can be employed by personnel with varying levels of education and experience [ 19, 20].
We thank P. Schweitzer and the staff at the Cornell DNA Sequencing and Genotyping Core Facility for sample preparation and sequencing and A. G. Clark and T. B. Sackton for helpful discussion.
We thank Travis Clark, Chelsea Baker and the Vanderbilt Technologies for Advanced Genomics core facility for RNA sample preparation and Illumina sequencing.
We thank the staff from the Genotyping Facilities at the Wellcome Trust Sanger Institute for sample preparation, Quality Control and Genotyping; Le Centre National de Génotypage, France; Duke University, North Carolina, USA; and the Finnish Institute of Molecular Medicine, Finnish Genome Center, University of Helsinki.
Remaining cDNA libraries were generated with the TruSeq RNA Sample Preparation Kit (Illumina) by Johns Hopkins Deep Sequencing and Microarray Core Facility (supplementary table S1, Supplementary Material online).
For details on sample preparation, data processing which was done at the Microarray DNA Facility of the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden and data analysis, see Supplemental Information.
These methods allow simultaneous analysis of several toxins; however, they require laboratory facilities, qualified personnel, as well as extensive cleanup procedures for sample preparation.
