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The minimal sample preparation employed in this study more closely reflects the native RNA population abundance compared with amplification-based, next-generation platforms.
Its popularity has increased due to the simple biological sample preparation employed when looking for metabolites, since the metabolic process generally results in the addition of polar groups to enhance elimination from the cellular tissue.
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High resolution MALDI MS in combination with solvent free sample preparation was employed to characterize a petroleum pitch and a coal tar pitch.
The sample preparation technique employed involves rapid direct derivatization (5 min) of acidified urine samples (25 μL) using a highly fluorinated phenyldiazomethane reagent [1- diazomethyl -3,5-bis trifluoromethyl benzene].
As imaging of living cells or tissues is often impractical with XFI, the possibility for some degree of chemical alteration from the in vivo state should always be considered, highlighting the importance for a thorough understanding of the likely chemical alterations, which may occur with the specific sample preparation protocol employed.
We thus conclude that the measured distance between the IM and OM, and hence the dimensions of the injectisome basal bodies being longer than isolated single particle structures, are unlikely to be a consequence of the cryo sample preparation method employed.
A sample preparation method employing the liquid--liquid extraction of YH-8 was also successfully developed.
In order to maximize the number of proteins that could be identified from each of the LB subfractions, multiple sample preparation strategies were employed prior to mass spectrometry.
Two different sample preparation procedures were employed.
In this study, two different sample preparation protocols were employed: Protocol A (for 36 of the 55 conditions) and Protocol B (for 19 of the 55).
Hydrogel preparation employing self-assembly of peptides offers facile biomimicry.
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