Exact(1)
Two milliliters of the supernatant were loaded onto a 1 g C18 sample preparation cartridge (Alltech Associates, Inc., Deerfield, IL).
Similar(59)
Cepheid's GeneXpert, for example, is an automated device for polymerase chain reaction (PCR) with special sample preparation cartridges for tuberculosis pathogens, MRSA or group B streptococci.
The system combines sample preparation in cartridges and the amplification and detection of nucleic acid DN in a fully integrated and automated instrument for analysis (Fig. 1).
The diluted sample (see Sample preparation) was loaded on to each cartridge, followed by low vacuum (1 min), and each cartridge was subsequently washed with 1 ml H2O, 1 ml 5% formic acid, 1 ml H2O and dried for 1 min. Each cartridge was then eluted with 1 ml of methanol-water (70 30) solution and with 1 ml of acetonitrile, followed by low vacuum (1 min).
For sample preparation, Sep Pack Alumina B cartridges (Waters, Milford, MA, USA) were used.
The results suggest that 2 ng/μl of hgDNA584 the fluid device could be extracted by tethered PNIPAAm brushes to be detected after PCR process, which could miniaturize cartridges of sample preparation for rapid disease diagnosis through fluid device to approach the lab-on-a-chip (LOC).
The chromatographic separation of estrogens (Fig. 4) which was performed after MISPE analysis using imprinted and non-imprinted materials revealed that the utilization of the MIP cartridge in the sample preparation step led to a substantial reduction of matrix interferences (chromatogram drawn with a solid line).
Solid-phase extraction with Oasis HLB extraction cartridges was used for sample preparation.
Having evaluated a number of SPE cartridges, we have now optimised sample preparation procedure for EADSA.
Supported liquid-phase extraction with Biotage SLE+ cartridges was used for the sample preparation.
Here, we present our findings and report an improved sample preparation procedure using polymeric hydrophilic lipophilic balanced reversed-phase cartridges.
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