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To validate our sample preparation approach, we prepared libraries from chicken HH16 heart tubes and whole embryos without heart tube.
A schematic view of the sample preparation approach is depicted in Figure 1.
Orthogonal array design was utilized to facilitate the optimization of the proposed sample preparation approach.
This established that sinapinic acid with the mixed sample preparation approach was the preferred method, which is in agreement with other studies [46,58].
We firstly incorporate a non-destructive sample preparation approach by using preservative media (ethanol) as the source of target DNA (hereafter referred to as ethanol-based DNA) and compare it with a conventional tissue-based DNA extraction (hereafter referred to as tissue-DNA).
The increase in proteome coverage from 35%to70%0% achieved by our study is likely due to the large number of replicates (eight total) compared to a maximum of three stated in the other studies, and the dual instrument, dual sample preparation approach used in our analysis.
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To overcome this problem, we had to change sample preparation approaches.
Novel sample preparation approaches for HPLC bioanalysis based on the phenomenon that acetonitrile can be separated from water by adding salts or cooling at subzero temperatures have been reported.
In this study we assessed 10 different matrices with 4 different sample preparation approaches.
Therefore, three sample preparation approaches were compared, including microwave-assisted digestion, acidification of the suspensions, as well as direct measurement via ICP-MS without further preparation.
Since previous XL-MS studies often cross-link proteins using a 1 1 mixture of nonlabeled and labeled cross-linkers to generate isotopic pairs, we wanted to compare whether equivalent results can be achieved using different sample preparation approaches.
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