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It was decided to sample plants for the proteomic analysis after 22 h and 8 days to evaluate both the early and late responses to gravitropic stimulation.
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All traits were measured from 5 randomly sampled plants for each accession after harvest.
Around 400 to 600 g of soil was taken from the holes remaining from each of the 10 sampled plants for white grub assessments (see above).
arachnitiformis (20); hybrids (13); O. lupercalis (20)) were labelled individually and sampled for chemical analyses of their floral odour; the same individuals were used to sample plant material for genetic analyses.
Nodules were harvested at 20-25 dafterfter inoculation; for these samples, plants were fertilized for the first seven days with nutrient solution containing 3.5 mM NO3 and subsequently fertilized every other day with a full nutrient solution lacking nitrogen.
The present study was part of a collaborative research program on the development of a FSPM for the sample plant (Hordeum vulgare L).
All sampled plants were monitored for infection with other pathogens.
For circadian samples, plants were grown in SD (8 h light/16 h dark) or LD (16 h light/8 h dark) conditions.
She photographed many of the specimens in their natural habitats, then dried and pressed samples of the plants for her collection.
Further, preliminary dialysis membrane calibration issues for sampling plant-available N compounds are discussed.
For the experiments with maize and Arabidopsis plants, three independent experiments were performed using six (if not otherwise specified) plants for each sample; each sample was measured performing three technical replicates.
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