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Each of the four colors associated with the Raman vibrational barcode of the four SEPs can be easily distinguished despite the complex biological background, while no significant signals were collected from WBCs in the sample or from cancer cells in the absence of SEPs.
Moreover, the accuracy of the exon length estimation for any species will increase if more data are available and if the ascertainment bias (e.g. resulting from overrepresentation of certain gene families in the sample, or from inclusion of only partially sequenced genes) is kept at the minimum.
DNA was derived from blood (discovery sample) or from cheek swabs (replication sample).
Germ-line DNA was prepared from either a matching blood sample or from normal tissue.
Germ-line DNA was prepared from either a matching blood sample or from normal tissue microdissected away from tumourtissue.
Unaffected DNA was obtained either from a blood sample or from microdissected endometrial tissue containing no tumour cells.
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Genomic DNA was isolated from silica-gel-dried samples or from herbarium specimens using the CTAB method47.
Polymerase chain reaction (PCR) amplifications were carried out either on DNA extracted directly from water samples or from isolates.
It is unclear whether the positive tests from the four Raiders came from newly provided urine samples or from those that were previously collected and stored at the U.C.L.A. lab.
This study is also limited in that the genomic and genotype data are not from the same samples or from the same area in Kenya, although malaria endemicity in both regions is similar24.
No viral RNA was detected in vaginal samples or from swab samples obtained from Subject 2 (low challenge dose).
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