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Duplicate hybridisations were performed for each RNA sample on different days using a different filter from the same manufactured lot.
Reproducibility has been achieved by performing replicate hybridizations of the same sample on different arrays [ 14, 17].
We performed three technical replicates for each sample on different days and then hybridised cRNA from caffeine-treated and untreated control samples to Illumina HumanHT-12 v3 gene expression arrays.
While these can in principle resolve the almost complete molecular composition in a sample on different levels (genomics, transciptomics, proteomics and metabolomics) they have to leave out the spatial domain.
After the two steps above, Parallel-META will parse the result of mapping, classify the 16S rRNA fragments of each sample, and then compare the construction of each sample on different biological level.
Technical replicates of the same sample on different nCounter runs were highly correlated (see Additional file 4), and variation between samples of different diagnoses was greater than that between samples of the same diagnosis in different nCounter runs (see Additional file 4), implying very little inter-run variability.
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Samples on different parts of the basin show distinct differences in heavy mineral compositions and they apparently divide into two groups according to the content of rutile (higher or lower than 4%).
For thermal cycling tests, an apparatus is developed to subject samples on different temperature cycle profiles.
Illustrated in Fig. 1 are the AFM images of the as-grown samples on different oriented GaAs surfaces.
Titania supported samples on different types of silica have been prepared through a sol gel method followed by hydrothermal processing.
Because two different samples are hybridized on a single microarray, and several microarrays are usually needed in a given experiment, there are many possible ways to combine samples on different microarrays.
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