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All incubations were done at room temperature, and all washings were performed with phosphate-buffered saline buffer (pH 7.5) Control sections used for determination of antibody reaction specificity: (a) tissue positive control (sections of mouse spleen) and (b) negative technique control (serial sections of each sample omitting the primary antibody).
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Although PSA clean-up method was selected as the most efficient for cleaning honey samples, omitting the clean-up step was the most economical approach with potential applicability in the food industry.
Negative controls have been performed in parallel for all samples omitting the reverse transcriptase in the RT reaction mix.
Accuracy is estimated as an average of classification successes calculated by including the sample omitted in the LOOCV round, and by evaluating its label (control or pain) by means of the SVM separation line.
Minus reverse transcriptase (NoRT) control reactions were prepared for each sample by omitting the Stratascript™ reverse transcriptase.
Initial experiments used Ovation RNA-Seq System V2 (NuGEN, San Carlos, CA) to amplify cDNA, but subsequent experiments proceeded directly to TruSeq (Illumina, San Diego, CA) from the rRNA-depleted sample but omitting the poly-A selection of mRNA.
Among them we chose the 3,332 and 3,648 samples, respectively, omitting the data that lack information on sales values.
Quantitative mass spectrometry targeting specific molecules rather than random sampling of the whole proteome, e.g., multiple reaction monitoring (MRM), offers an efficient and effective means to multiplex the measurement of several candidates in patient samples, thereby omitting the need for antibodies prior to clinical assay design.
If the removal of a single outlier did not improve the SD of the replicate Cts for a sample (≤0.2), the assay was repeated or the sample omitted.
The sampled received signal, omitting the symbol-level details and replacing by the chip level, can be expressed as r p = ∑ k = 1 K ∑ l = 1 L c l p a k p − τ l T c + n k, l p, (1).
Negative samples were performed omitting the first antibody.
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