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A separate sample of wheat straw was swelled in 4 M potassium hydroxide over 2 hours at ambient temperature at a liquor to solid ratio of 20 1, in order to extract a hemicellulose-rich fraction.
In a sample of wheat germplasm consisting of 52 diverse genotypes, null alleles for EST-SSRs occurred at a frequency of 45% [ 39] which is higher compared to the result obtained here.
Although 73% of samples had levels of OTA above the LOD (which ranged from 0.001 ng/g 0.008 ng/g, depending on the food stuff), the highest measured OTA value from the Quebec city survey was a sample of raisins containing 2.3 ng OTA/g and the highest measured value from the Calgary survey was a sample of wheat flour containing 1.7 ng/g, both of which are below the proposed ML.
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A few weeks later, 128 crates of seeds holding around 38,000 samples of wheat, barley, lentil, chickpea, fava bean, pea, grass pea and legumes were removed from the vault located in the frigid, snow-packed archipelago halfway between Norway and the north pole.
Samples of wheat, barley, and grasses have been requested, the only thing left is the paperwork.
This was demonstrated by the specific detection of Fusarium cerealis in grain samples of wheat.
Field samples of wheat and leafhopper collected from different regions of China were detected by both real-time PCR and gel-based PCR.
Six samples of wheat (Triticum aestivum, variety Kosack) from field trials, three grown in conventional farming systems and three in organic farming systems (biodynamic and ecological), were roller- and stone-milled, respectively.
Historical samples of wheat and barley from 1957 to 2000 similarly showed a strong prevalence of M. majus over M. nivale indicating that M. majus has been the main prevalent Microdochium species in Danish cereals for at least 50 years.
Thirty samples of wheat milling by-products (wheat bran, wheat middlings, wheat shorts, wheat red dog, wheat feed flour), collected from 11 flour mills, were fed to growing pigs to determine their digestible energy (DE) and metabolizable energy (ME) content and to establish equations for predicting their DE and ME content based on chemical analysis.
Quantitative real-time PCR assays were designed to separate the two Microdochium species based on the translation elongation factor 1α gene (TEF-1α) and used to analyse a total of 374 seed samples of wheat, barley, triticale, rye and oat sampled from farmers' fields across Denmark from 2003 to 2007.
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