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As with other types of blood analyses, enzyme assays have been automated with autoanalyzers, which make it possible to obtain data on the serum activity of up to 20 or more enzymes simultaneously on one sample of serum.
Instrumental linearity was verified in the range of 1.00 500 ng/mL, corresponding to a concentration range of 0.0300 15.0 μg/mL in 50 μL sample of serum matrix and alternate matrix.
After administration of a load of 20 gr of proteins by enteral route, a creatinine clearance (CrCl) was calculated for each of the next 6 hours, with a timely collection of urine and a sample of serum creatinine at the end of every period.
Prior to inoculation, a sample of serum was taken from each TgOvPrP4 mouse.
Briefly, each sample of serum, 200 µl, was pipetted to a pre-cleaned 8 ml glass test tube, and spiked with 13C-labelled p,p'-DDE and CB-153 internal standards.
But, for three of them, the sample of serum was not properly handled.
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Samples of serum and saliva were collected on the same day.
From a subset of the PTLD cases (N=12) and a series of corresponding stem-cell recipient controls (N=36), consecutive samples of serum (N=103 and 364, respectively) were studied by qPCR for EBV-DNA, and the clinical data were reviewed.
These methods were subsequently used to positively identify both C. forsteri and C. orientalis DNA in preserved samples of serum, gill, and heart from ranched Southern Bluefin Tuna Thunnus maccoyii.
Sampling of serum was carried out from non-fasting individuals.
b15 samples of serum were analyzed per group.
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